静岡大学若手グローバル研究リーダー育成拠点  
大西研究室 Division of Global Readers, Shizuoka University
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静岡大学

 
創造科学技術大学院

若手グローバル研究
リーダー育成拠点


農学部

  応用生物化学科

  天然物化学教室

GRLバイオ
サイエンスセミナー
所在地
静岡大学
若手グローバル研究
リーダー育成拠点
〒422-8529
静岡県静岡市駿河区大谷836

dtonish (at) ipc.shizuoka.ac.jp
 
 
 
14) Dong F, Yang Z, Baldermann S, Kajitani Y, Ota S, Kasuga H, Imazeki Y, Ohnishi T, Watanabe N (2012) Characterization of L-phenylalanine metabolism to acetophenone and 1-phenylethanol in the flowers of Camellia sinensis using stable isotope labeling.
Journal of Plant Physiology, in press

13) Seki H, Sawai S, Ohyama K, Mizutani M, Ohnishi T, Sudo H, Fukushima EO, Akashi T, Aoki T, Saito K, Muranaka T (2011) Triterpene Functional Genomics in Licorice for Identification of CYP72A154 Involved in the Biosynthesis of Glycyrrhizin.
The Plant Cell. in press

12) Hamberger B, Ohnishi T, Hamberger B, Seguin A, Bohlmann J. (2011) Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyses multiple oxidations in resin acid biosynthesis of conifer defense against insects.
Plant Physiology,

11) Todoroki Y, Narita K, Muramatsu T, Shimomura H, Ohnishi T, Mizutani M, Ueno K, Hirai N. (2011) Synthesis and biological activity of amino acid conjugates of abscisic acid.
Bioorg Med Chem.19, 1743-1750

10) Okazaki M, Nimitkeatkai H, Muramatsu T, Aoyama H, Ueno K, Mizutani M, Hirai N, Kondo S, Ohnishi T, Todoroki Y. (2011) Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A.
Bioorg Med Chem. 19, 406-413.

9) Chen XM, Kobayashi H, Sakai M, Hirata H, Asai T, Ohnishi T, Baldermann S, Watanabe N. Functional characterization of rose phenylacetaldehyde reductase(PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol. (2011)
Journal of Plant Physiology, 168, 88-95

8) Ohnishi T, Yokota T, Mizutani M. (2009) Insights into the function and evolution of P450s in plant steroid metabolism.
Phytochemistry, 70, 1918-1929

7) Seki, H., Ohyama, K., Sawai, S., Mizutani, M., Ohnishi, T., Sudo, H., Akashi, T., Aoki,T., Saito, K., Muranaka, T. (2008) Licorice β-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin.
Proc. Natl. Acad. Sci. U S A. 105, 14204-14209

6) Sekimata, K., Ohnishi, T., Mizutani, M., Todoroki, Y., Han, S.Y., Uzawa, J., Fujioka, S., Yoneyama, K., Takeuchi, Y., Takatsuto, S., Sakata, K., Yoshida, S., Asami, T. (2008) Brz220 Interacts with DWF4, a Cytochrome P450 Monooxygenase in Brassinosteroid Biosynthesis, and Exerts Biological Activity.
Biosci. Biotechnol. Biochem., 72, 7-12

5) Ohnishi, T., Szatmari, A. M., Watanabe, B., Fujita, S., Bancos, S., Koncz, C., Lafos, M., Shibata, K., Yokota, T., Sakata, K., Szekeres, M., Mizutani, M. (2006) C-23 Hydroxylation by Arabidopsis CYP90C1 and CYP90D1 Reveals a Novel Shortcut in Brassinosteroid Biosynthesis.
The Plant Cell, 18, 3275-3288

4) Ohnishi, T., Watanabe, B., Nomura, T., Ohta, D., Yokota, T., Sakata, K., Mizutani, M.(2006) Tomato cytochrome P450 CYP734A7 functions in brassinosteroid catabolism.
Phytochemistry, 67, 1895-1906

3) Ohnishi, T., Watanabe, B., Sakata, K., Mizutani, M. (2006) Enzymatic Analysis of Tomato Cytochrome P450 CYP724B2 involved in Brassinosteroids Biosynthesis.
Biosci. Biotechnol. Biochem., 70, 2071-2080

2) Sakamoto, T., Morinaka, Y., Ohnishi, T., Sunohara, H., Fujioka, S., Ueguchi-Tanaka, M., Mizutani, M., Sakata, K., Takatsuto, S., Yoshida, S., Tanaka, H., Kitano, H., Matsuoka, M. (2006) Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice.
Nature Biotechnology, 24, 105-109

1) Fujita, S., Ohnishi, T., Watanabe, B., Yokota, T., Takatsuto, S., Fujioka, S., Yoshida, S., Sakata, K., Mizutani, M. (2006) Arabidopsis CYP90B1 catalyzes the early C-22 hydroxylation of C27, C28, and C29 sterols.
The Plant Journal, 45, 765-774
  
   Proceedings of International Conferences
11)  Ohnishi, T., Hamberger, B., Hamberger, B., Bohlman, J.
Biochemial chacterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka Spruce.
9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites. 25th-29th May, 2009

10) Hamberger, B., Ohnishi, T., Hamberger, B., Bohlman, J.
Gene structure and expression if the cytochrome P450 CYP720B family in sitka spruce (Picea sitchensis).
9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites. 25th-29th May, 2009

9)  Ohnishi, T., Hamberger, B., Bohlman, J.
Expression and biochemical characterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka spruce.
Banff Conference on Plant Metabolism,
Banff, Canada, pp.23, July, 2008

8) Hamberger, B., Ohnishi, T., Bohlman, J.
Functional genomics of cytochrome P450 in conifer diterpene resin acid formation.
Banff Conference on Plant Metabolism,
Banff, Canada, pp.10, July, 2008

7) Ohnishi, T., Hamberger, B., Bohlman, J.
Expression in insect cell and E. coli and biochemical characterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka spruce. 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology,
Nice, France, pp.35, June, 2008

6) Hamberger, B., Ohnishi, T., Bohlman, J.
Cytochrome P450 mono-oxygenases in conifer defence: Functional characterization of the CYP720B subfamily in Sitka spruce (Picea sitchensis) diterpene resin acid biosynthesis. 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, pp.34,
Nice, France, June, 2008

5) Bohlmann, Breuil, C., Diguistini, S., Hamberger, B., Keeling, C.I., Lippert, D., Ohnishi, T., Ralph, S., Reid, K.
Conifer Defence and Biomaterials: The Role of Terpenoid Synthases and Cytochrome P450s in Diterpene Resin Acid Biosynthesis and Diversification of Oleoresin Terpenoids. 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology,
Nice, France, June, 2008.

4) Hamberger, B., Ohnishi, T., Bohlman, J.
Functional genomics of cytochrome P450s in conifer defence: characterization of the CYP720B subfamily in diterpene resin acid formation in Sitka spruce. Forest Biology Symposium, -Winter 2008, pp.13,
Victoria, Canada, February, 2008

3) Ohnishi, T., Bancos, S., Watanabe, B., Yokota, T., Sakata, K., Szekeres, M., Mizutani, M.Functional characterization of Arabidopsis CYP90C1 and CYP90D1 encode Brassinosteroids C-23 hydroxylazes.
8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, pp.13, Swansea, Wales, July, 2006.

2) Ohnishi, T, Watanabe, B., Sakata, K., M., Mizutani, M.
CYP724Bs and CYP90Bs are redundant C-22 hydroxylases in the early C-22 hydroxylation routes of brassinosteroid biosynthesis. 
8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, pp.14, Swansea, Wales, July, 2006

1) Ohnishi, T., Nakamura, M., Nagatani, A., Yokota, T., Sakata, K., Mizutani, M.
Analysis of brassinosteroids metabolites catalyzed by Arabidopsis CHIBI2 (CYP72C1).
18th International Conference on Plant Growth Substances, pp.117,
Canberra, Australia, September, 2004 
  
4) Good Presentation award "Biochemial chacterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka Spruce''
9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites. 29th May, 2009

3) Paper Award “Enzymatic Analysis of Tomato Cytochrome P450 CYP724B2 involved in Brassinosteroids Biosynthesis.”
Japan Society for Bioscience, Biotechnology, and Agrochemistry, 24 March 2007

2) Poster Award “Biochemical characterization of brassinosteroid C-3 oxidase”
The Japanese Society for Chemical Regulation of Plants, 30 October 2006

1) Poster Award “Functional characterization of Arabidopsis CYP90C1 and CYP90D1 encode Brassinosteroids C-23 hydroxylazes”.
8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 27 July 2006
  
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